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es cell clones  (Zymo Research)


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    Structured Review

    Zymo Research es cell clones
    Es Cell Clones, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/es+cell+clones/Quick-DNA+96+Plus+Kit/pm40403729-1036-7-13
    Average 95 stars, based on 152 article reviews
    es cell clones - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types
    Article Snippet: .. The targeting vector (30 μg of DNA) was then co-electroporated with a plasmid containing a mouse codon optimized Bxb1 gene under the control of the cytomegalovirus (CMV) promoter (100 μg of DNA) into the Bxb1-landing pad ES cell line and following hygromycin drug selection at 100-150 μg/ml for 5 days, monoclonal populations of cells were hand-picked and expanded. gDNA was prepared from the modified ES cell clones using a kit (Zymo Research Cat#D4071) and it was screened by qPCR and junctional PCR assays to confirm proper targeting into the TIGRE locus. ..

    Control:

    Article Title: A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types
    Article Snippet: .. The targeting vector (30 μg of DNA) was then co-electroporated with a plasmid containing a mouse codon optimized Bxb1 gene under the control of the cytomegalovirus (CMV) promoter (100 μg of DNA) into the Bxb1-landing pad ES cell line and following hygromycin drug selection at 100-150 μg/ml for 5 days, monoclonal populations of cells were hand-picked and expanded. gDNA was prepared from the modified ES cell clones using a kit (Zymo Research Cat#D4071) and it was screened by qPCR and junctional PCR assays to confirm proper targeting into the TIGRE locus. ..

    Selection:

    Article Title: A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types
    Article Snippet: .. The targeting vector (30 μg of DNA) was then co-electroporated with a plasmid containing a mouse codon optimized Bxb1 gene under the control of the cytomegalovirus (CMV) promoter (100 μg of DNA) into the Bxb1-landing pad ES cell line and following hygromycin drug selection at 100-150 μg/ml for 5 days, monoclonal populations of cells were hand-picked and expanded. gDNA was prepared from the modified ES cell clones using a kit (Zymo Research Cat#D4071) and it was screened by qPCR and junctional PCR assays to confirm proper targeting into the TIGRE locus. ..

    Modification:

    Article Title: A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types
    Article Snippet: .. The targeting vector (30 μg of DNA) was then co-electroporated with a plasmid containing a mouse codon optimized Bxb1 gene under the control of the cytomegalovirus (CMV) promoter (100 μg of DNA) into the Bxb1-landing pad ES cell line and following hygromycin drug selection at 100-150 μg/ml for 5 days, monoclonal populations of cells were hand-picked and expanded. gDNA was prepared from the modified ES cell clones using a kit (Zymo Research Cat#D4071) and it was screened by qPCR and junctional PCR assays to confirm proper targeting into the TIGRE locus. ..

    Article Title: A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types.
    Article Snippet: Each targeting vector (30 μg of DNA) was then co-electroporated with a plasmid containing a mouse codon optimized Bxb1 gene under the control of the cytomegalovirus (CMV) promoter (100 μg of DNA) into the Bxb1-landing pad ES cell line and following hygromycin drug selection at 100–150 μg/mL for 5 days, monoclonal populations of cells were hand-picked and expanded. .. Genomic DNA was prepared from the modified ES cell clones using a kit (Zymo Research CatD4071) and it was screened by qPCR and junctional PCR assays to confirm proper targeting into the TIGRE locus. ..

    Clone Assay:

    Article Title: A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types
    Article Snippet: .. The targeting vector (30 μg of DNA) was then co-electroporated with a plasmid containing a mouse codon optimized Bxb1 gene under the control of the cytomegalovirus (CMV) promoter (100 μg of DNA) into the Bxb1-landing pad ES cell line and following hygromycin drug selection at 100-150 μg/ml for 5 days, monoclonal populations of cells were hand-picked and expanded. gDNA was prepared from the modified ES cell clones using a kit (Zymo Research Cat#D4071) and it was screened by qPCR and junctional PCR assays to confirm proper targeting into the TIGRE locus. ..

    Article Title: A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types.
    Article Snippet: Each targeting vector (30 μg of DNA) was then co-electroporated with a plasmid containing a mouse codon optimized Bxb1 gene under the control of the cytomegalovirus (CMV) promoter (100 μg of DNA) into the Bxb1-landing pad ES cell line and following hygromycin drug selection at 100–150 μg/mL for 5 days, monoclonal populations of cells were hand-picked and expanded. .. Genomic DNA was prepared from the modified ES cell clones using a kit (Zymo Research CatD4071) and it was screened by qPCR and junctional PCR assays to confirm proper targeting into the TIGRE locus. ..

    Real-time Polymerase Chain Reaction:

    Article Title: A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types
    Article Snippet: .. The targeting vector (30 μg of DNA) was then co-electroporated with a plasmid containing a mouse codon optimized Bxb1 gene under the control of the cytomegalovirus (CMV) promoter (100 μg of DNA) into the Bxb1-landing pad ES cell line and following hygromycin drug selection at 100-150 μg/ml for 5 days, monoclonal populations of cells were hand-picked and expanded. gDNA was prepared from the modified ES cell clones using a kit (Zymo Research Cat#D4071) and it was screened by qPCR and junctional PCR assays to confirm proper targeting into the TIGRE locus. ..

    Article Title: A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types.
    Article Snippet: Each targeting vector (30 μg of DNA) was then co-electroporated with a plasmid containing a mouse codon optimized Bxb1 gene under the control of the cytomegalovirus (CMV) promoter (100 μg of DNA) into the Bxb1-landing pad ES cell line and following hygromycin drug selection at 100–150 μg/mL for 5 days, monoclonal populations of cells were hand-picked and expanded. .. Genomic DNA was prepared from the modified ES cell clones using a kit (Zymo Research CatD4071) and it was screened by qPCR and junctional PCR assays to confirm proper targeting into the TIGRE locus. ..

    Polymerase Chain Reaction:

    Article Title: A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types
    Article Snippet: .. The targeting vector (30 μg of DNA) was then co-electroporated with a plasmid containing a mouse codon optimized Bxb1 gene under the control of the cytomegalovirus (CMV) promoter (100 μg of DNA) into the Bxb1-landing pad ES cell line and following hygromycin drug selection at 100-150 μg/ml for 5 days, monoclonal populations of cells were hand-picked and expanded. gDNA was prepared from the modified ES cell clones using a kit (Zymo Research Cat#D4071) and it was screened by qPCR and junctional PCR assays to confirm proper targeting into the TIGRE locus. ..

    Article Title: A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types.
    Article Snippet: Each targeting vector (30 μg of DNA) was then co-electroporated with a plasmid containing a mouse codon optimized Bxb1 gene under the control of the cytomegalovirus (CMV) promoter (100 μg of DNA) into the Bxb1-landing pad ES cell line and following hygromycin drug selection at 100–150 μg/mL for 5 days, monoclonal populations of cells were hand-picked and expanded. .. Genomic DNA was prepared from the modified ES cell clones using a kit (Zymo Research CatD4071) and it was screened by qPCR and junctional PCR assays to confirm proper targeting into the TIGRE locus. ..



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